METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES
摘要
The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
申请公布号
EP1427851(A4)
申请公布日期
2005.08.10
申请号
EP20020757473
申请日期
2002.08.28
申请人
ARIZONA BOARD OF REGENTS
发明人
WILLIAMS, PETER;TAYLOR, THOMAS, J.;WILLIAMS, DANIEL, J., B.;GOULD, IAN;HAYES, MARK, A.