PROCESS FOR MEASURING AMOUNT OF ENDOTOXIN OR (1 3)-.BETA.-D-GLUCAN BY KINETIC TURBIDIMETRIC METHOD

摘要

A process for measuring the amount of endotoxin or (1 .fwdarw. 3)-.beta.-D-glucan (referred to hereinafter as .beta.- glucan) contained in a sample, which comprises mixing the sample with amebocyte lysate of horseshoe crabs in the presence of at least one water-soluble polymer selected from the group consisting of a polyethylene glycol, a polyvinyl alcohol, a methyl cellulose and a hydroxypropyl cellulose, applying a light to the resulting mixture, measuring the time required until a degree of optical variation of the resulting mixture reaches a predetermined value after the mixing of the sample with the amebocyte lysate, or after the lapse of the predetermined time from the mixing of the sample with the amebocyte lysate, and determining the amount of endotoxin or .beta.-glucan contained in the sample on the basis of the relationship between the said time and the amount of endotoxin or .beta.-glucan; and also a reagent for measuring the amount of endotoxin or .beta.- glucan contained in a sample, which comprises amebocyte lysate of horseshoe crabs and at least one water-soluble polymer selected from the group consisting of a polyethylene glycol, a polyvinyl alcohol, a methyl cellulose and a hydroxypropyl cellulose.