发明名称 |
ANTI-LAWSONIA INTRACELLULARIS VACCINES AND DIAGNOSTIC AGENTS |
摘要 |
Cultivating Lawsonia intracellularis (LI) bacteria comprises: (a) incubating the bacteria at an oxygen concn. of 18% and (b) maintaining the bacteria in suspension, to cultivate the bacteria. Also claimed are: (1) a method for cultivating LI bacteria comprising: (a') inoculating culture cells with an inoculum comprising LI bacteria so as to infect the cells with the bacteria and (b') incubating the infected cells at a temp. of 36 -38 [deg]C in an oxygen concn. of 0-8% while agitating the infected cells so as to cultivate the LI while maintaining the infected cells in suspension; (2) a method for producing an attenuated LI strain comprising: (i) obtaining culture cells infected with LI bacteria, (ii) incubating the infected cells at an oxygen concn. of 0-18%, (iii) agitating the infected cells so as to cultivate the bacteria while maintaining the infected cells in suspension, (iv) passaging at least a portion of the cultivated bacteria and (v) selecting for an attenuated strain to provide an attenuated LI bacteria; (3) a vaccine for inducing an immune response to LI bacteria in an animal comprising an attenuated strain of LI in a carrier, where the attenuated strain is in amt. effective to produce an immune response. |
申请公布号 |
PL189187(B1) |
申请公布日期 |
2005.07.29 |
申请号 |
PL19960358164 |
申请日期 |
1996.06.05 |
申请人 |
BOEHRINGER INGELHEIM/NOBL LABORATORIES INC. |
发明人 |
KNITTEL JEFFREY P.;ROOF MICHAEL B. |
分类号 |
G01N33/554;A61K39/02;A61K39/106;A61K39/40;A61P31/04;C07K16/12;C12N1/00;C12N1/20;C12N1/36;C12N5/06;C12N9/92;C12Q1/02;G01N33/569 |
主分类号 |
G01N33/554 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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