TRANSGLUTAMINASE HAS INTRINSIC KINASE ACTIVITY

摘要

A method of identifying compounds capable of modulating trans-glutaminase (TG) kinase activity is described. The method involves adding a test compound to a mixture of TG and a suitable TG kinase substrate, incubating t he mixture under conditions promoting TG kinase activity, and determining if th e test compound activates or modulates TG kinase activity as indicated by greater than expected TG-mediated phosphorylation of the TG kinase substrate or if the te st compound inhibits TG kinase activity as indicated by lower than expected TG- mediated phosphorylation of the TG kinase substrate as compared to a control comprising TG and a suitable TG kinase substrate incubated under conditions promoting TG kinase activity.