| 摘要 |
Methods and systems for expanding the dynamic range of a system are provided . One method includes splitting fluorescent light emitted by a particle into multiple light paths having different intensities, detecting the fluorescent light in the multiple light paths with different channels to generate multip le signals, and determining which of the channels is operating in a linear rang e based on the multiple signals. The method also includes altering the signal generated by the channel operating in the linear rang to compensate for the different intensities. Another method includes illuminating a particle in multiple illumination zones with light having different intensities and separately detecting fluorescent light emitted by the particle while located in the multiple illumination zones to generate multiple signals. The method also includes determining which of the signals is located in a linear range and altering the signal located in the linear range to compensate for the different intensities.
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