发明名称 |
Methods of purification of cytochrome p450 proteins and of their crystallizing |
摘要 |
The invention provides a method for the purification of cytochrome P450 molecules, the method comprising expressing in a host cell culture a cytochrome P450 molecule; recovering said cells from said culture and suspending said cells in a high salt buffer; lysing said cells and removing cell debris to provide a high-salt lysate; adding to said lysate a detergent to provide a high-salt-detergent lysate; and recovering said P450 from said lysate. The method provides yields of P450 proteins suitable for crystallization.
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申请公布号 |
US2005164341(A1) |
申请公布日期 |
2005.07.28 |
申请号 |
US20050516338 |
申请日期 |
2005.03.15 |
申请人 |
COSME JOSE;WARD ALISON;VUILLARD LAURENT;WILLIAMS PAMELA;HAMILTON BRUCE |
发明人 |
COSME JOSE;WARD ALISON;VUILLARD LAURENT;WILLIAMS PAMELA;HAMILTON BRUCE |
分类号 |
G01N23/20;C07K1/22;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/02;C12N15/09;C12N15/53;(IPC1-7):C12N9/02 |
主分类号 |
G01N23/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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