发明名称 |
Method and polynucleotides for determining translational efficiency of a codon |
摘要 |
A method is disclosed for determining the translational efficiency of an individual codon in a cell. The method includes introducing into the cell a synthetic construct including a reporter polynucleotide fused in frame with a tandem repeat of the individual codon, wherein the reporter polynucleotide encodes a reporter protein, and wherein the synthetic construct is operably linked to a regulatory polynucleotide; and measuring expression of the reporter protein in the cell to determine the translational efficiency of the codon.
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申请公布号 |
US2005166282(A1) |
申请公布日期 |
2005.07.28 |
申请号 |
US20050041806 |
申请日期 |
2005.01.24 |
申请人 |
THE UNIVERSITY OF QUEENSLAND |
发明人 |
FRAZER IAN H.;ZHOU JIAN;SUN XIAO Y. |
分类号 |
C12N15/09;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12Q1/02;C12Q1/68;(IPC1-7):A01H1/00;C12N15/82 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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