发明名称 |
METHOD OF EXPRESSING PROTEIN HAVING UNNATURAL AMINO ACID INTEGRATED THEREINTO |
摘要 |
The present invention discloses an expression method for non-naturally-occurring amino acid-containing protein comprising: expressing in animal cells: (A) a mutant tyrosyl-tRNA synthetase that is a mutation of tyrosyl-tRNA synthetase originating in E. coli with an enhanced specificity for a non-naturally-occurring tyrosine derivative as compared with the specificity for tyrosine; (B)suppressor tRNA originating in Bacillus species, Mycoplasma species or Staphylococcus species of eubacteria and capable of binding with the tyrosine derivative in the presence of the mutant tyrosyl tRNA synthetase; and, (C) a desired protein gene that has undergone a nonsense mutation at a desired site; wherein, the tyrosine derivative is incorporated at the location of this nonsense mutation.
|
申请公布号 |
EP1557469(A1) |
申请公布日期 |
2005.07.27 |
申请号 |
EP20030809875 |
申请日期 |
2003.10.31 |
申请人 |
RIKEN;JAPAN SCIENCE AND TECHNOLOGY AGENCY |
发明人 |
YOKOYAMA, SHIGEYUKI;SHIROUZU, MIKAKO;SAKAMOTO, AYAKO;SAKAMOTO, KENSAKU |
分类号 |
A61K48/00;C12N9/00;(IPC1-7):C12N15/85;C12N5/10 |
主分类号 |
A61K48/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|