TRANSLATIONAL FUSION PARTNERS FOR THE SECRETORY PRODUCTION OF PROTEINS

摘要

A novel translational fusion partner(TFP) is provided to express and secret a non-producible protein which is hard to be recombinantly produced. A TFP protein inducing the secretion of recombinant protein interleukin-2, colony stimulating factor(G-CSF) or lipase B(CalB) from eukaryotic cells is selected from the group consisting of (a) a TFP1 protein having 90-99.9% of homology to an amino acid sequence described as SEQ ID : NO. 1 and an amino acid sequence having the TFP1 protein activity (b) a TFP2 protein having 90-99.9% of homology to an amino acid sequence described as SEQ ID : NO. 3 and an amino acid sequence having the TFP2 protein activity (c) a TFP3 protein having 90-99.9% of homology to an amino acid sequence described as SEQ ID : NO. 5 and an amino acid sequence having the TFP3 protein activity (d) a TFP4 protein having 90-99.9% of homology to an amino acid sequence described as SEQ ID : NO. 7 and an amino acid sequence having the TFP4 protein activity (e) a TFP1-3 protein having 90-99.9% of homology to an amino acid sequence described as SEQ ID : NO. 9 and an amino acid sequence having the TFP1-3 protein activity (f) a TFP1-4 protein having 90-99.9% of homology to an amino acid sequence described as SEQ ID : NO. 10 and an amino acid sequence having the TFP1-4 protein activity (g) a TFP3-1-1 protein having 90-99.9% of homology to an amino acid sequence described as SEQ ID : NO. 40 and an amino acid sequence having the TFP1-4 protein activity and (h) a TFP3-1-2 protein having 90-99.9% of homology to an amino acid sequence described as SEQ ID : NO. 42 and an amino acid sequence having the TFP3-1-2 protein activity.