Yeast transformation vector containing auxotrophic dominant gene yeast transfomant containing it and their preparation

摘要

A yeast transformation vector containing a dominant auxotrophic gene, a yeast transformant containing the same and a method of preparation thereof. An adenine auxotrophic transformant able to grow in the presence of adenine can be obtained by inducing a dominant adenine auxotrophic (DAD) mnutation, isolating the dominant adenine auxotrophic gene DAD1, DNA sequencing the DAD1 gene to determine its mutation site, constructing pCABIOD101 vector containing the DAD1 gene (accession number: KCTC 1013BP), and transforming haploid laboratory yeast strains and diploid industrial yeast strains respectively with the pCABIOD101 vector. Therefore, the transformation vector system of the present invention can be used in improving existing industrial yeasts and various microorganisms.