发明名称 |
VERFAHREN ZUR REINIGUNG VON MENSCHLICHEM INTERLEUKIN-1 REZEPTOR ANTAGONIST AUS REKOMBINANTEN E.COLI |
摘要 |
A process for purifying human Interleukin-1 receptor antagonist (IL-1ra), obtained by fermenting a strain of recombinant E. coli, which comprises: a) loading the mixture to be purified, which has been buffered at a pH value lower than 6.2 and optionally diluted to reduce its ionic strength at a low value, onto a cationic exchange matrix and eluting said matrix with an aqueous buffered solution having a pH value from about 7.5 to 9.0 and a low ionic strength; and b) applying the eluate from the cationic exchange step, containing the desired IL-1ra protein, directly onto an anionic exchange matrix and eluting with an aqueous buffered solution having a pH value within the above range 7.5 to 9.0 and an increased ionic strength. |
申请公布号 |
DE69730041(T2) |
申请公布日期 |
2005.07.21 |
申请号 |
DE1997630041T |
申请日期 |
1997.05.21 |
申请人 |
AVENTIS BULK S.P.A., MAILAND/MILANO |
发明人 |
ZANETTE, DINO;SARUBBI, GIACOMO;SOFFIENTINI, ADOLFO |
分类号 |
C12N15/09;C07K1/18;C07K14/54;C12N1/21;(IPC1-7):C07K14/54 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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