发明名称 |
Process for the preparation of a desired erythropoietin glyco-isoform profile |
摘要 |
The present invention provides a process for the production of erythropoietin (EPO) with high purity and with a desired profile of EPO glycol-isoforms by using a combination of specific chromatographic steps in such a manner that the starting EPO glycol-isoform profile is changed or modified. The applied chromatographic steps includes at least (a) dye affinity chromatography, and (b) hydrophobic chromatography and/or (c) anion-exchange chromatography. In a preferred embodiment, the process further includes (d) gel filtration chromatography. The present invention also provides a process for the determination of erythropoietin (EPO) glycol-isoform profile in an EPO containing composition.
|
申请公布号 |
US2005153879(A1) |
申请公布日期 |
2005.07.14 |
申请号 |
US20040508598 |
申请日期 |
2004.09.22 |
申请人 |
SVETINA MONICA;SVETEK JELKA;GANTAR-KSELA MATEJA;BRINC MATJAZ;FRANCKY ANDREJ |
发明人 |
SVETINA MONICA;SVETEK JELKA;GANTAR-KSELA MATEJA;BRINC MATJAZ;FRANCKY ANDREJ |
分类号 |
G01N33/483;A61K38/00;B01J20/281;C07K1/18;C07K1/20;C07K1/22;C07K14/505;C12P21/02;G01N27/447;G01N30/46;G01N30/88;G01N33/53;(IPC1-7):A61K38/18 |
主分类号 |
G01N33/483 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|