The present invention provides methods and compositions for conferring selective death on cells expressing a specific target precursor messenger RNA (selective target pre-mRNA). The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with a target precursor messenger RNA molecule (target pre-mRMA) expressed within a cell and mediate a trans-splicing reaction resulting in the generation of a novel chimeric mRNA molecule (chimeric mRNA) capable of encoding a light producing protein or enzyme. Cell death is further mediated by the presence of a photosensitizer which upon photoactivation produces cytotoxicity.
申请公布号
WO2005023990(A3)
申请公布日期
2005.07.14
申请号
WO2004US29022
申请日期
2004.09.07
申请人
INTRONN, INC.;MITCHELL, LLOYD, G.;OTTO, EDWARD;MERRIL, CARL, R.