发明名称 |
Biopolymer array substrate thickness dependent automated focus-distance determination method for biopolymer array scanners |
摘要 |
A method and system for determining a biopolymer array substrate thickness dependent optimal focus distance for scanning a molecular array by a molecular array scanner are provided. A reference substrate is automatically scanned at successively greater distances of the stage from a light gathering medium, such as an optical fiber, or z-positions, to produce data providing a functional relationship between z-position and measured signal intensities. The data is then processed by an array substrate thickness dependent focus-finding routine that selects an optimal focus-distance for data scans which is optimized for the thickness of the reference substrate. Also provided are methods of determining the thickness of a biopolymer array substrate using a position sensitive device (PSD) component of a biopolymer array scanner. Further methods include determining the thickness of said biopolymeric array and automatically selecting an optimal focus distance using the determined thickness and a calibration function on thickness versus optimal focus distance. The subject invention finds use in a variety of different applications, including both genomic and proteomic applications.
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申请公布号 |
US2005153349(A1) |
申请公布日期 |
2005.07.14 |
申请号 |
US20040022302 |
申请日期 |
2004.12.23 |
申请人 |
GHOSH JAYATI;CORSON JOHN F.;SILLMAN DEBRA A. |
发明人 |
GHOSH JAYATI;CORSON JOHN F.;SILLMAN DEBRA A. |
分类号 |
G01N21/27;G01N21/64;G02B21/24;(IPC1-7):C12Q1/68;G06F19/00;G01N33/48;G01N33/50 |
主分类号 |
G01N21/27 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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