| 摘要 |
A primary mammalian cell which may be infected with a pathogen is cultivated under reduced oxygen to improve susceptibility to infection with a pathogen, pathogen propagation, and/or to change or maintain a degree of differentiation of the cell in a predetermined manner. In preferred aspects, the cell is a hepatocyte, optionally infected with HCV, or a blastomere from a single-cell biopsied blastocyst. Such biopsied cell may then be expanded to a population of stem cells under concurrent cultivation of the blastocyst, which is then used for IVF implantation.
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