REAGENT SYSTEMS FOR BIOLOGICAL ASSAYS AND THE PRODUCTION OF ARRAYS

摘要

A reagent system for printing, assaying, and processing nucleic acid microarrays is provided. The system comprises: a printing kit, which includes a nucleic acid spotting solution; and a hybridization kit, which includes a nucleic acid pre-hybridization solution, a nucleic acid hybridization solution, and first, second, and third wash reagents, wherein the respective constituent components of the printing and hybridization kits are stable and retain functional performance, when stored together at a temperature between about 10<C to about 50?C. A background reducing agent or solution is also included. The present reagent solutions are optimized for use with glass substrates having preferably an amine-coating, such as GAPS