发明名称 |
METHODS TO INCREASE NUCLEOTIDE SIGNALS BY RAMAN SCATTERING |
摘要 |
The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid 13 . Exonuclease 15 treatment of the labeled nucleic acid 13 results in the release of labeled nucleotides 16, 130 , which are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides 16, 130 released from a nucleic acid 13 by exonuclease 15 treatment are covalently cross-linked to silver or gold nanoparticles 140 and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus 10, 100, 210 for nucleic acid sequencing. |
申请公布号 |
EP1488002(A4) |
申请公布日期 |
2005.07.13 |
申请号 |
EP20030719382 |
申请日期 |
2003.03.11 |
申请人 |
INTEL CORPORATION |
发明人 |
SU, XING;BERLIN, ANDREW;KOO, TAE-WOONG;CHAN, SELENA;SUNDARARAJAN, NARAYAN;YAMAKAWA, MINEO |
分类号 |
G01N21/65;B01L3/00;C12M1/00;C12Q1/68;G01N33/543 |
主分类号 |
G01N21/65 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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