A member of a new class of bacterial toxin has been isolated and characterised. The bacterial toxin is of the AB5 type and is characterised in that it has a subtilase domain. It is cytotoxic to Vero cells, and toxicity in vivo in mice occurs in a number of different sites. Mutation of the serine active residue results in greater than 99% reduction in activity. The protein has been purified and antibodies have been prepared for both the A subunit and B subunits, and ELISA detection methods have been developed. The nucleic acid sequence has been determined and primers specific for the toxin have been used for a preliminary screen of a range of patient samples to ascertain the extent to which the toxin is present.
申请公布号
WO2005061714(A1)
申请公布日期
2005.07.07
申请号
WO2004AU01796
申请日期
2004.12.21
申请人
ADELAIDE RESEARCH AND INNOVATION PTY LTD;PATON, ADRIENNE, WEBSTER;PATON, JAMES, CLELAND