发明名称 |
Hybrid plasmid pzzsa coding the synthesis of angiogenis of angiogenin protein and escherichia coli bl21(des) pzzsa strain as the the superoducer of the recombinant chimeric protein of human angiogenin |
摘要 |
The invention relates to biotechnology and enhances the expression efficiency of the hybrid gene and stability of the angiogenin protein, as well as provides a possibility of affinity purification of chimeric protein on IgG sorbents. The hybrid plasmid pZZSA with a molecular weight of 3.814 megadalton (MDa) (6192 bp) which drives the synthesis of the chimeric protein of angiogenin is disclosed, comprising an XhoI/EcoRI fragment of the pGM280 plasmid DNA comprising a tandem of E. coli tryptophan operon promoters, a lambda phage transcription terminator, a beta-lactamase gene and an ori replication initiation site; an EcoRI/EcoNI fragment of the PfM plasmid comprising a synthetic translation enhancer of the bacteriophage T7 gene and gene Ap coding for the amino acid sequence corresponding to 3988-4845 bp; synthetic chimeric angiogenin gene (Ang) fused with Spa; unique recognition sites for restriction nucleases. The strain Escherichia coli BL21 (DE3) pZZSA (the Collection of Microorganisms of the Interregional Center for Human Microcenosis Correction, Novosibirsk, Registration No. MCKM B-127) that is the superproducer of recombinant chimeric protein of human angiogenin is described.
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申请公布号 |
US2005148061(A1) |
申请公布日期 |
2005.07.07 |
申请号 |
US20040502554 |
申请日期 |
2004.07.22 |
申请人 |
RAMAZANOV YURY A.;METVETSOV NIKOLAI P.;MAISTRENKO VELENTINA F. |
发明人 |
RAMAZANOV YURY A.;METVETSOV NIKOLAI P.;MAISTRENKO VELENTINA F. |
分类号 |
C12N15/70;C07K14/515;C07K19/00;C12N1/21;C12N15/12;C12N15/62;C12R1/19;(IPC1-7):C12N1/21;C12N15/74 |
主分类号 |
C12N15/70 |
代理机构 |
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代理人 |
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