<p>An interstitial collagenase is disclosed which acts as an ATP-independent molecular motor driven by the proteolysis of substrate collagen. Also disclosed is a method of reacting interstitial collagenase with a collagen fibril to encounter multiple cleavage sites without substantial dissociation of said interstitial collagenase and thereby act as a molecular motor driven by the proteolysis of substrate collagen. The invention thus is useful as a research tool in the tissue remodeling and cell- matrix interaction. The invention also has significant utility in the screening and development of inhibitors of interstitial collagenase and as a research tool for evaluating the activity and effect of drug candidates.</p>
申请公布号
WO2005061700(A1)
申请公布日期
2005.07.07
申请号
WO2004US38236
申请日期
2004.12.09
申请人
WASHINGTON UNIVERSITY;GOLDBERG, GREGORY;SAFFARIAN, SAVEEZ;COLLIER, IVAN, E.;MARMER, BARRY, L.;ELSON, ELLIOT, L.
发明人
GOLDBERG, GREGORY;SAFFARIAN, SAVEEZ;COLLIER, IVAN, E.;MARMER, BARRY, L.;ELSON, ELLIOT, L.