发明名称 |
Method for producing a recombinant polypeptide. |
摘要 |
The invention relates to a method for producing a transformed eukaryotic host cell which expresses a recombinant polypeptide of interest which method comprises introducing into a eukaryotic host cell: (a) a first polynucleotide vector which comprises (i) a first nucleotide sequence which encodes a recombinant polypeptide of interest, and (ii) a second nucleotide sequence encoding a selectable marker, which second nucleotide sequence is amplified when the host cell is contacted with a selection agent; and (b) a second polynucleotide vector having essentially the same nucleotide sequence as the first polynucleotide vector except that the second nucleotide sequence is replaced with a third nucleotide sequence which encodes a different selectable marker; the first polynucleotide vector and second polynucleotide vector being stably integrated into the genome of the host cell. |
申请公布号 |
ZA200403802(B) |
申请公布日期 |
2005.07.01 |
申请号 |
ZA20040003802 |
申请日期 |
2004.05.18 |
申请人 |
SANDOZ GMBH. |
发明人 |
KURT SCHOERGENDORFER;JOERG WINDISCH;RENATE KUNERT;FLORIAN UNTERLUGGAUER |
分类号 |
C12N15/09;A61K38/22;A61P7/00;A61P7/06;C07K14/505;C12N5/10;C12N15/85;C12N15/90;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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