发明名称 |
IMPROVED METHODS FOR PROTEIN INTERACTION DETERMINATION |
摘要 |
The present invention provides a method for identifying a plurality of pairs of interacting proteins and plasmids for use in the method. The pair of plasmids is adapted for use in a modified two hybrid system wherein wherein each plasmid comprises a recombinase recognition site. The method comprises the steps of providing cDNAs encoding test polypeptides, inserting the cDNAs into the first and second plasmids, recombining the first and second plasmids to obtain recombined plasmids, isolating and digesting the recombined plasmids, ligating the restriction fragments to a universal adapter to provide a pool of digested fragments flanked by a universal adapter, selecting and amplifying desired sequences, forming concatamers from the amplified sequences, and sequencing the concatamers to determine the nucleotide sequences encoding a plurality of pairs of interacting proteins. |
申请公布号 |
WO2004102157(A3) |
申请公布日期 |
2005.06.30 |
申请号 |
WO2004US14613 |
申请日期 |
2004.05.10 |
申请人 |
HEALTH RESEARCH INC.;PRUITT, STEVEN, C.;HASTIE, ALEXANDER;MIELNICKI, LAWRENCE |
发明人 |
PRUITT, STEVEN, C.;HASTIE, ALEXANDER;MIELNICKI, LAWRENCE |
分类号 |
C12N15/10;C12N15/85;C12P19/34;C12Q1/68;G01N;G01N33/53 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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