NOVEL MIXTURES FOR ASSAYING NUCLEIC ACID, NOVEL METHOD OF ASSAYING NUCLEIC ACID WITH THE USE OF THE SAME AND NUCLEIC ACID PROBE TO BE USED THEREFOR

摘要

[PROBLEMS] To provide novel mixtures for assaying a target nucleic acid characterized by enabling a nucleic acid assay while: 1) requiring no step of diluting the target nucleic acid; and 2) requiring no procedure of changing probe concentration depending on the concentration of the target nucleic acid. [MEANS FOR SOLVING PROBLEMS] 1) A mixture which comprises one internal standard nucleic acid and two nucleic acid probes labeled with one fluorescent dye; 2) a mixture for measuring Km value which comprises one internal standard nucleic acid having a partial mutation and one nucleic acid probe labeled with one fluorescent dye; 3) a mixture which comprises one internal standard nucleic acid and a double-nucleic acid probe labeled with two fluorescent dyes; and a method of assaying a nucleic acid with the use of these mixtures.