| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for measuring a DNA methylation ratio, capable of using an optional region as a target sequence, without preliminarily analyzing a methylation site, and capable of simply and accurately measuring the methylation ratio of the cytosine of a DNA, and to provide a method for evaluating a state of a cell. SOLUTION: This method for measuring the DNA methylation ratio comprises (1) a process of treating the DNA containing the measuring target base sequence with a cytosine modifier which transforms the cytosine into another base, (2) a process of conducting a DNA amplification operation of the treated DNA, by using a chain containing the transformed base sequence derived from the measuring target base sequence as a template, in the presence of a labeling dCTP, (3) a process of separating a DNA chain containing the amplified base sequence corresponding to the transformed base sequence from the amplified DNA, and (4) a process of measuring an amount of the labeled cytosine in the separated DNA chain. The method for evaluating the state of the cell comprises conducting contact of a chromosome sample of the cell with a methylation-resistant cytosine antibody under a divalent-bonding condition. COPYRIGHT: (C)2005,JPO&NCIPI
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