DUAL HYBRIDIZATION OF COMPLEX NUCLEIC ACID SAMPLES FOR SEQUENCING AND SINGLE-NUCLEOTIDE POLYMORPHISM IDENTIFICATION

摘要

The present invention is drawn to a method of nucleic acid sequencing by hybridizing a selection probe with a conserved region of a target nucleic acid molecule that contains a sequence area of interest; hybridizing a sequencing probe that is different from the selection probe to a variable region of the target nucleic acid molecule; determining the nucleic acid sequence of the area of interest only. The present invention is for the identification and analysis of single nucleotide polymorphisms (SNP).