发明名称 |
BACTERIA WITH REDUCED GENOME |
摘要 |
The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to 14% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence. |
申请公布号 |
ZA200405509(B) |
申请公布日期 |
2005.06.29 |
申请号 |
ZA20040005509 |
申请日期 |
2004.07.12 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
Frederick R BLATTNER;Gyorgy POSFAI;Christopher D HERRING;Guy PLUNKETT;Jeremy D GLASNER;Trevor Martin TWOSE |
分类号 |
C12N;C12N15/09;C12N1/08;C12N1/20;C12N1/21;C12N15/10;C12N15/70;C12N15/90;C12P21/02;C12R1/19 |
主分类号 |
C12N |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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