发明名称 |
METHOD OF TARGETED GENE DISRUPTION, GENOME OF HYPERTHERMOSTABLE BACTERIUM AND GENOME CHIP USING THE SAME |
摘要 |
It is intended to provide an efficient and sure gene targeting method embodied at an arbitrary position in the genome of an organism and a kit therefor. It is also intended to provide a method for targeted-disruption of an arbitrary gene in the genome of an organism which comprises: 1) the step of providing the whole sequencial data of the genome of the organism; 2) the step of selecting at least one arbitrary region in the sequence; 3) the step of providing a vector containing a sequence homologous with the region selected above and a marker gene; 4) the step of transforming the organism by the vector; and 5) the step of providing the organism under such conditions as allowing homologous recombination. Moreover, the genome of a hyperthermostable bacterium and its array are provided. <IMAGE>
|
申请公布号 |
EP1548105(A1) |
申请公布日期 |
2005.06.29 |
申请号 |
EP20030793947 |
申请日期 |
2003.08.29 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY AGENCY |
发明人 |
IMANAKA, TADAYUKI;ATOMI, HARUYUKI |
分类号 |
C07K14/195;C12Q1/02;C12Q1/68;(IPC1-7):C12N15/00 |
主分类号 |
C07K14/195 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|