Nucleic acid labeling method by PCR

摘要

It is provided a nucleic acid labeling method that can reduce to a minimum the amount of a labeling substance used and can effectively incorporate the labeling substance into a product, a target nucleic acid to be detected. In the labeling of a target nucleic acid sequence in a product while it is amplified by PCR, at least one type from among four types of substrates A, T, C and G is chosen as a substrate to be labeled, the concentration of the chosen substrates is adjusted to be lower than that of the non-chosen substrates, and at least the labeled substrates are contained in the chosen substrates.