| 摘要 |
Provided is a method for producing a continuous cell line capable of supporting the growth of a Marek's disease virus (MDV) strain comprising infecting or transfecting a cell with a vector comprising a nucleic acid or fragment thereof of an MDV strain and culturing the cell or a progeny thereof under conditions suitable for the expression of the nucleic acid and propagation of the cell or progeny. The nucleic acid may comprise an MDV glycoprotein gE gene or functional fragment. Also provided is a method of generating, isolating, and/or maintaining a hypervirulent, very virulent, very virulent plus, virulent, and/or avirulent MDV strain comprising infecting a described cell with the MDV strain or strains, and culturing the cell under conditions suitable for the propagation of the cell and the generation, maintenance and isolation of the MDV strain or strains. Also provided is a method to prepare a vaccine capable of inducing protection against disease, preferably an MDV associated disease in an avian, including culturing a continuous cell according to the invention and harvesting cell culture components therefrom. |
