| 摘要 |
<p>The procedure consists of setting up a standard range of proteins responsible for proteinic break, taking a sample of the must or wine to be analysed, applying drops of each point in the protein range and the sample to strips of nitrocellulose and leaving to dry. The protein fixing sites are then saturated and the strips are washed, and the washed strips are placed in contact with primary polyclonal antibodies directed against the proteinic break proteins. The strips are washed again and placed in contact with secondary antibodies coupled to a peroxydase and directed against the primary antibodies, and after washing again they are placed in a revealing solution to produce a black protein precipitate which is compared visually with the stains on the corresponding sample sites and the standard range to determine the quantity of proteins responsible for the proteinic break.</p> |
