Xylanase variants having altered sensitivity to xylanase inhibitors caused by mutations in the lid region

摘要

Disclosed are methods for altering the sensitivity of a xylanase polypeptide to an inhibitor comprising modifying at amino acids 11, 12, 13, 15, 17, 29, 31, 32, 34, 116, 117, 119, 120, 121, 122, 123, 124 or 175 of B. subtilis amino acid sequence or equivalent position in a homologous xylanase, then measuring the sensitivity and comparing to the sensitivity of the parent enzyme. In addition, modified xylanase polypeptides are also provided, that have both a modification in the amino acid at position 11, as well as another modified amino acids at one of the positions listed above.