Differentiating aggregated molecules from unaggregated normal molecules

摘要

A method for detecting aggregation or polymerization of molecules in a sample, which method comprises the steps of: a) exposing a sample containing the molecules to at least two labelling moieties which will bind to only a single mutually exclusive site on each of the molecules; and b) determining whether multiply-bound labelling moieties are present. The method is particularly of use in differentiating pathogenic aggregated protein molecules from non-pathogenic, non-aggregated protein molecules.