发明名称 |
Method of genetic modification of a wild type viral sequence |
摘要 |
The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence, comprising the following successive steps: submitting said sequence to point mutation(s) which allow the substitution of at least one amino-acid into a different amino-acid; selecting genetically modified TGB-3 wild type viral sequences having said point mutation(s) and which are not able to promote cell-to-cell movement of a mutant virus having a dysfunctional TGB-3 wild type viral sequence, when expressed in trans from a replicon; further selecting among said genetically modified TGB-3 viral sequences, the specifically genetically modified sequence which inhibits infection with a co-inoculated wild type virus when the mutant form was expressed from a replicon; and recovering said specifically genetically modified TGB-3 viral sequence.
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申请公布号 |
US2005138687(A1) |
申请公布日期 |
2005.06.23 |
申请号 |
US20040980146 |
申请日期 |
2004.11.01 |
申请人 |
LAUBER EMMANUELLE;GUILLEY HUBERT;RICHARDS KEN;JONARD GERARD |
发明人 |
LAUBER EMMANUELLE;GUILLEY HUBERT;RICHARDS KEN;JONARD GERARD |
分类号 |
A01H5/00;C07K14/08;C12N5/10;C12N15/82;C12Q1/68;(IPC1-7):C12Q1/70;A01H1/00 |
主分类号 |
A01H5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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