| 摘要 |
The invention relates to a method for identifying methylated DNA against a background of unmethylated DNA. According to said method, the DNA double strands are split and subsequently reassociated to form hemimethylated double strands. The hemimethylated DNA is then converted using enzymes into completely methylated DNA. The yield of methylated DNA is thus increased. The methylated DNA can then be analysed by means of various methods. The inventive method is particularly suitable for the diagnosis and prognosis of carcinosis and other diseases associated with the modification of the methylation status, in addition to the prediction of undesired medicament activity. |
