CRYSTAL OF OXIDIZED LDL-RECOGNITION DOMAIN OF OXIDIZED LDL RECEPTOR LOX-1, STEREOSTRUCTURE THEREOF AND UTILIZATION OF THE SAME
摘要
<p>In order to clarify the binding manner of an oxidized LDL receptor LOX-1 to oxidized LDL in detail, it is intended to clarify the stereostructure of the oxidized LDL-binding domain of LOX-1. In particular, it is finally intended to clarify the stereostructure of a CTLD-binding domain as a dimer structure holding the same pattern as occurring on the cell surface layer. The problem of clarifying the oxidized LDL-binding domain (CTLD: C-type lectin-like domain) of LOX-1 employed in crystallization and a protein containing the LOX-1 oxidized LDL-binding domain and an NECK domain (a region connecting a transmembrane part to the CTLD) occurring as a dimer can be solved by obtaining various crystals with the use of samples obtained by unwinding proteins expressed on a mass scale in Escherichia coli in accordance with the method disclosed in the description (preferably comprising the step of adding a buffer solution having a buffering region in the acidic pH range to a solution and the step of adding calcium or zinc ion to the solution, or the step of adding a buffer solution having a buffering region in the neutral pH range to the solution in the case of the LOX-1 dimer) and analyzing the thus obtained crystals.</p>
申请公布号
WO2005054460(A1)
申请公布日期
2005.06.16
申请号
WO2004JP17890
申请日期
2004.12.01
申请人
OHKI, IZURU;BIOMOLECULAR ENGINEERING RESEARCH INSTITUTE;ISHIGAKI, TOMOKO;OYAMA, TAKUJI;MORIKAWA, KOSUKE;TATE, SHIN-ICHI