发明名称 |
Fast method of transforming competent cells |
摘要 |
A fast method of transforming competent cells is described. The competent cells are thawed at room temperature or in a water bath. Plasmid DNAs and competent cells are mixed together, then the mixture is subject to heat shock treatment. After plating the mixture on a low-temperature selective medium by a low-temperature plating tool, the competent cells are cultured on the selective medium.
|
申请公布号 |
US2005130289(A1) |
申请公布日期 |
2005.06.16 |
申请号 |
US20050043739 |
申请日期 |
2005.01.26 |
申请人 |
YEASTERN BIOTECH CO., LTD. |
发明人 |
CHEN TZU-CHIH;HUA WEI-NI |
分类号 |
C12N15/09;C12M1/00;C12M1/02;C12M1/26;C12N1/21;C12N15/64;C12N15/70;C12N15/74;C12N15/87;C12R1/19;(IPC1-7):C12N1/21 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|