发明名称 |
Splint-assisted enzymatic synthesis of polyribounucleotides |
摘要 |
The present invention comprises methods and compositions for splint-assisted enzymatic synthesis of polyribonucleotides using an RNA polymerizing enzyme. The invention provides ligating ribonucleotides comprising ligating a donor RNA molecule to an acceptor RNA molecule in the presence of RNA ligase and a splint, wherein the donor RNA molecule is comprised of at least one nucleotide and a ligation linker moiety, the acceptor RNA molecule is comprised of at least one nucleotide and a ligation linker moiety and the splint is comprised of a polyribonucleotide. The invention also provides splints for use in splint-assisted enzymatic synthesis using an RNA polymerizing enzyme.
|
申请公布号 |
US2005130201(A1) |
申请公布日期 |
2005.06.16 |
申请号 |
US20040965547 |
申请日期 |
2004.10.13 |
申请人 |
DHARMACON, INC. |
发明人 |
DERAS MICHAEL;PLEISS JEFFREY A.;SCARINGE STEPHEN |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|