| 摘要 |
It is intended to provide a process for producing a virus vector, which contains a membrane protein having an activity of binding to sialic acid in its envelope, by using neuraminidase (NA) originating in a gram- positive bacterium. This process involves the step of culturing cells capable of producing the virus vector in the presence of NA originating in the gram-positive bacterium and then collecting the thus produced virus. According to this process, a virus having a high titer can be produced at a high cost performance. The thus produced virus vector is capable of highly efficiently transferring a gene into cells to which a gene can be hardly transferred by the existing methods (for example, blood cells and hematopoietic cells including hematopoietic stem cells, cells having mucus including mucosal epithelial cells and so on) and, therefore, is useful as a vector for gene therapy.
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