发明名称 |
SINGLE CHAIN ANTIBODY AND UTILIZATION THEREOF |
摘要 |
The present invention provides a single chain antibody that retains its original specific binding activity with an antigen, and a labeled single chain antibody in which a labeling substance is bound to the single chain antibody. Specifically, the labeled single chain antibody of the present invention can be produced by linking a labeling substance to a linker part of a single chain antibody. The antibody is produced using a wheat embryo-derived cell-free protein synthesis system, and production is carried out in a low reductive state that allows an intramolecular disulfide bond to be retained. Further, bonding the antibody to a solid phase via the labeling substance enables production of an immobilized single chain antibody as well as a method for analyzing an antigen-antibody reaction using the immobilized single chain antibody.
|
申请公布号 |
EP1541588(A1) |
申请公布日期 |
2005.06.15 |
申请号 |
EP20030765324 |
申请日期 |
2003.07.18 |
申请人 |
CELLFREE SCIENCES CO., LTD. |
发明人 |
ENDO, YAETA;KAWASAKI, TAKAYASU;SAWASAKI, TATSUYA |
分类号 |
C07K16/00;C07K16/12;(IPC1-7):C07K16/00;C07K19/00;C12N15/09;G01N33/53 |
主分类号 |
C07K16/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|