| 摘要 |
The present invention provides methods for selecting artificial promotor candidates that reduce the number of promotors required to be evaluated by actual experimentation when designing artificial promotor candidates that are potentially effective for structural genes. In these artificial promotor candidate selection methods, a randomly selected nucleotide sequence is joined upstream of a structural gene (S 1 ). Next, nucleotides are extracted in a predetermined pattern from a designated region containing at least the transcription start site of the joined nucleotide sequence, thereby generating a virtual amino acid sequence, and an index curve is generated from the virtual amino acid sequence (S 2 , S 3 ). Then, the nucleotide sequence joined upstream of the structural gene is selected as an artificial promotor candidate if the index curve exhibits a sign change near the transcription start site (S 4 ).
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