Method for detecting hybridized nucleic acid with improved sensitivity

摘要

The present invention provides a method for improving detection sensitivity of hybridized nucleic acid immobilized on a solid support of sensing device for gene assay, by removing non-hybridized nucleic acid probe using nuclease from the solid support. In accordance with the invented method, background signal caused by single stranded probe that is not hybridized with target nucleic acid or signal caused by non-specific binding of target nucleic acid to probe is decreased or removed, which improves detection sensitivity of hybridization with a high accuracy, and minimizes the loss of hybridized nucleic acid in the course of washing background signal removed in the conventional method.