| 摘要 |
FIELD: biotechnology, in particular immobilized biocatalysts. ^ SUBSTANCE: invention relates to biocatalysts based on microorganism cells, incorporated in gel carrier matrix for microbiological lactic acid production. As immobilized biocatalyst matrix polymer-based cryogel in used, wherein polymer is selected from group containing polyvinyl alcohol, polygalactomannan, amylolytically stable starch, gelatin. Ratio of starting components (mass %) is: biomass of microorganism cells with lactate dehydrogenase activity 0.001-4.0 (calculated as dry material); polymer 2-15; and balance: water/aqueous buffer. Immobilized catalyst is obtained by biomass incorporating into cryogel matrix followed by immobilized cell treatment by incubation at 28-45°C for 10-200 h in medium capable of increasing lactate dehydrogenase activity of immobilized biomass. Lactic acid is obtained by substrate solution treatment with said immobilized biocatalyst at pH 5.5-6.5 and 28-45°C for 15-24 h followed by target product isolation by known methods. ^ EFFECT: strength carrier with high porosity; biomass with increased activity; effective and safe method for lactic acid production. ^ 3 cl, 7 ex |
