A L-sorbosone dehydrogenase as well as the encoding gene and the use thereof

摘要

The present invention discloses a L-sorbosone dehydrogenase as well as the encoding gene and the use thereof. The L-sorbosone dehydrogenase provided by the invention is the protein with the amino acid residue sequence of SEQ ID NO:2 in sequence listing or the protein derived from SEQ ID NO:2, and sharing at least 80% homology with the amino acid residue sequence of SEQ ID NO:2 in sequence listing and having the same activity with SEQ ID NO:2. The gene encoding for the L-sorbosone dehydrogenase is one of the following nucleotide sequences: 1)SEQ ID NO:1 in sequence listing; 2) the polynucleotide encoding the protein sequence of SEQ ID NO:2 in sequence listing; 3)the DNA sequence sharing aboye 80% homology with the DNA sequence defined by SEQ ID NO:1 in sequence listing and encoding the saine functional protein. When the E. coli engineering strain carried the gene encoding for the L-sorbosone dehydrogenase of the invention is co-cultivated with the bacteria converting 2-KGA, the rSNDH of the invention can significantly improve the efficiency of conversion to 2-KGA .