发明名称 |
Novel dehydrogenase and a gene encoding the same |
摘要 |
The object of the present invention is to provide a novel dehydrogenase having a property which is different from that of known dehydrogenases. The present invention provides a dehydrogenase having the following physicochemical properties: (1) effect: to produce N-alkyl-L-alanine from pyruvic acid and alkylamine or dialkylamine using NADPH and/or NADH as coenzyme; (2) substrate specificity: to show activity to alkylamine or dialkylamine but not to ammonium; (3) optimal pH when using phenylpyruvic acid and methylamine as substrates is around 10; and (4) when treated at 30° C. for 30 minutes, the enzyme is stable at around pH 5 to 10.5.
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申请公布号 |
US2005124040(A1) |
申请公布日期 |
2005.06.09 |
申请号 |
US20040927028 |
申请日期 |
2004.08.27 |
申请人 |
MITSUBISHI CHEMICAL CORPORATION |
发明人 |
ESAKI NOBUYOSHI;MIHARA HISAAKI;HARA MARI;UEDA MAKOTO |
分类号 |
C07H21/04;C12N1/15;C12N1/19;C12N1/21;C12N9/02;C12N9/06;C12N15/31;C12P13/04;C12P13/06;(IPC1-7):C12N9/06 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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