发明名称 |
METHODS FOR PRODUCING BIOLOGICAL SUBSTANCES IN PIGMENT-DEFICIENT MUTANTS OF BACILLUS CELLS |
摘要 |
The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Bacillus cell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parent Bacillus cell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants of Bacillus cells and methods for producing the mutants. |
申请公布号 |
EP1537229(A2) |
申请公布日期 |
2005.06.08 |
申请号 |
EP20030771884 |
申请日期 |
2003.07.25 |
申请人 |
NOVOZYMES BIOTECH, INC. |
发明人 |
TANG, MARIA;SLOMA, ALAN;STERNBERG, DAVID;BEHR, REGINE |
分类号 |
C12N1/12;C07K14/32;C12N1/20;C12N1/21;C12N9/02;C12P1/04;C12P19/00;C12P21/02;C12P21/04;C12P21/06 |
主分类号 |
C12N1/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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