| 摘要 |
The invention provides a vaccine composition and a method of preparation including the steps of: forming a water-in-oil emulsion including an alginate in water, an oil, an antigen, and a surfactant composition comprising a methyl cellulose ether, polyoxyethylene (20) sorbitan trioleate and a poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer having 16, 21 30 or 39 PO residues surfactant in a concentration of 1 and/or 2 ml per 10 ml of 1.5 % (w/v) alginate solution, or a surfactant composition comprising polyoxyethylene (20) sorbitan trioleate and poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer having 30 PO residues surfactant in a concentration of 2 ml per 8 ml of 1.5% (w/v) alginate solution; the mean volume particle size of the alginate microparticles being about 6 mu m or less before sonication; followed by crosslinking the alginate in the emulsion with at least two cations selected from the group consisting of aluminum, barium, calcium, lithium, manganese, strontium, and zinc, to form antigen-containing, crosslinked alginate microparticles; and harvesting the microparticles. The compositions of the invention have improved antigen loading, reduced microparticle size, increased hydrophobicity, improved uptake by antigen sampling cells, controlled antigen release characteristics, and improved immunogenicity. |
