Method for introducing nucleic acids and other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electric current

摘要

Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors, each connected to a high voltage source. - Apparatus for electrophoretic transfer of nucleic acids, peptides, proteins and/or other biologically active molecules into eukaryotic cells comprises two capacitors (7, 8), each connected to a high voltage source (5, 6). Each capacitor discharges via a power transistor (9, 10) into a cuvette holding the cell sample. The first capacitor is charged with a preset voltage U1 and the second with a voltage U2 given by the equation: - U2 = R x I2 x K2, - R1 = the resistance of the cuvette and suspension in it - I2 = the desired current - K2 = a correction factor based on the properties of thee cuvette - ACTIVITY - None given. - MECHANISM OF ACTION - Gene therapy. - No supporting data provided.