METHOD FOR OBTAINING MODIFIED IMMUNOGLOBULINS WITH REDUCED IMMUNOGENICITY OF MURINE ANTIBODY VARIABLE DOMAINS AND COMPOSITIONS CONTAINING THEM

摘要

A method for identifying differences in mammalian species specific amino acid residues onto T-cell antigenic sequences on an immunoglobulin includes comparing the framework amino acids of a variable domain of a first mammalian species with the variables domains of a second mammalian species; determining the subgroups of the second mammalian species to which the first mammalian species most closely corresponds; determining the second mammalian species sequence which is most similar to the first mammalian species sequence; identifying amino acid residues of the first mammalian species which differ from the amino acid residues of the second mammalian species, with said amino acids being onto T-cell antigenic sequences in the variable region of the immunoglobulins; and identifying only those amino acid residues which are not within a complementarity region or are not directly involved with canonical structures or Vernier zone.