| 摘要 |
The present invention provides, inter alia, methods for the isolation and purification of defensin polypeptides from white blood cells. More particularly, the present invention provides methods for the extraction and purification of defensin polypeptides, i.e., a-defensins 1, 2 and 3, from intact white blood cells, without the need for nitrogen cavitation (Parr bomb) or mechanical disruption, centrifugation and gel filtration. |
