发明名称 |
Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices |
摘要 |
A method is provided for the detection or quantitation of a target sequence of a nucleic acid molecule in a sample, comprising: a) contacting the sample with a non-nucleotide probe which will hybridise to at least a portion of the target sequence to form a non-nucleotide probe/ target sequence complex; b) contacting the sample with a matrix wherein said nucleic acid molecule will electrostatically bind to the matrix and wherein the backbone of the non-nucleotide probe or probes is sufficiently neutral or positively charged, under electrostatic binding conditions, that it exhibits little or no affinity for the matrix; c) contacting the sample with an enzyme capable of degrading sample contaminants, including the nucleic acid molecule but not the non-nucleotide probe/target sequence complex, at a time after performing step (a); and d) detecting or quantitating the non-nucleotide probe or the non-nucleotide probe/target sequence complex at a time after performing step (b), as a means to detect or quantitate the target sequence in the sample. <??>A kit and a composition for use in such a method are also provided.
|
申请公布号 |
EP1536023(A1) |
申请公布日期 |
2005.06.01 |
申请号 |
EP20040014435 |
申请日期 |
1999.12.08 |
申请人 |
BOSTON PROBES, INC. |
发明人 |
JOHANSEN, JACK T.;HYLDIG-NIELSEN, JENS J.;FIANDACA, MARK J.;COULL, JAMES M. |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|